The purpose of this assessment is to provide vital information for the embryologists as to which ART treatment (IVF or ICSI) to be applied after egg retrieval.
This assessment evaluates parameters included in a Semen Analysis and the ability of the spermatozoa to withstand in-vitro stress tests. In the in-vitro stress tests, the freshly ejaculated semen sample is prepared through high-speed centrifugation and exposure to room temperature (normal body temperature is 37oC).
With normal or above average semen sample, it is easy to obtain motile sperm by a wide spectrum of sperm preparation techniques. However, for abnormal semen sample a modified preparation technique must be carefully carried out in order to obtain adequate amount of live and motile sperm, to maximize the chances of fertilization and to provide as many normally fertilized oocytes as possible for embryo transfer or cryopreservation.
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| Test tube containing semen sample being processed using high speed centrifugation. |
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| Test tube after high speed centrifugation with sperm pellet at the bottom of the tube. |
Despite this sometimes we are still unsure of the fertilizing capability of the sperm. The sperm is then left overnight (Sperm Survival Test – SST) to assess its survival. Occasionally, sperm DNA fragmentation test may be advised which provides some information on the potential outcome of fertility treatment.